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Image Search Results
Journal: Journal of Neurotrauma
Article Title: Kir6.2, the Pore-Forming Subunit of ATP-Sensitive K + Channels, Is Overexpressed in Human Posttraumatic Brain Contusions
doi: 10.1089/neu.2017.5619
Figure Lengend Snippet: Antibody validation. (A) Upper images show the result of a western blot analysis of human Kir6.2 in five specimens of human brain tissue (lanes 1 to 5: 2, 4 corresponding to contusion specimens and 1, 3, 5 corresponding to healthy tissue) using two independent antibodies (Alomone Ab and GeneTex Ab). The bar graphs are shown as a visual aid for the inmunoblot results. Optical density with both antibodies was compared and resulted in a Pearson correlation coefficient of 0.88 (p = 0.049). The scatterplot is shown as a visual aid to the immunoblot results. (B) Fluorescence labeling of Kir6.2 using Alomone Ab (red) and SantaCruz Ab (green); merged images in yellow. (C) Western blot analysis of a human recombinant Kir6.2 protein (ab114436; Abcam, Cambridge, UK) (1) tagged with green fluorescent protein (GFP) and GFP recombinant protein (2) used as a negative control. (D) Kir6.2 expression (green) in a cell line of human cardiomyocytes (P10451; Innoprot, Derio, Spain) used as a positive control. Nuclei were counterstained with 4,6-diamino-2-phenylindole (DAPI).
Article Snippet: The scatterplot is shown as a visual aid to the immunoblot results. (B) Fluorescence labeling of Kir6.2 using Alomone Ab (red) and SantaCruz Ab (green); merged images in yellow. (C) Western blot analysis of a human recombinant Kir6.2 protein (ab114436; Abcam, Cambridge, UK) (1) tagged with green fluorescent protein (GFP) and GFP recombinant protein (2) used as a negative control. (D) Kir6.2 expression (green) in a cell line of human
Techniques: Biomarker Discovery, Western Blot, Fluorescence, Labeling, Recombinant, Negative Control, Expressing, Positive Control